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. 2020 Dec 3;13(1):389–410. doi: 10.18632/aging.202144

Figure 1.

Figure 1

Downregulation of DOK3 reduces the levels of inflammatory cytokines in microglia. Microglia were infected with a DOK3-specific short hairpin RNA (shRNA) to establish stably down-regulated DOK3 expression. Total cell lysates of DOK3-shRNA and Scr-shRNA microglia were used to determine the levels of mRNA for DOK3 (A), TNF-α (B), IL-1β (C), IL-6 (D), iNOS (E), P38 (F), CD68 (G), CD11B (H), Arg1 (I), and CD206 (J) by quantitative reverse transcription-polymerase chain reaction (RT-qPCR). N=3 per group, and data are presented as means ± SEM. *p < 0.05, #p < 0.01 vs. Scr-shRNA.