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. 2020 Dec 3;13(1):163–180. doi: 10.18632/aging.202301

Figure 4.

Figure 4

Relative expression of apoptotic factors determined by quantitative real time PCR (qRT-PCR) analysis of (A) Tumour necrosis factor-α (TNF-α), (B) Tumour suppressor (P53), (C) Apoptosis regulator (BAX), (D) Apoptotic protease activating factor 1 (APAF 1), (E) Cytochrome-c and (F) Caspase 3 genes in Jurkat cells treated with 10μm Compound C02 for 24 h. mRNA levels of apoptotic factors were determined relative to the endogenous control Actin, according to the formula 2 to the power of delta cycle threshold (2DCt), where DCt¼Ct, reference gene – Ct, test gene. Differences between experimental groups were tested for significance using nonparametric Mann–Whitney test (GraphPad Prism version 5, San Diego, CA), for both mRNA, protein expressions and other analysis. Levels of significance are indicated by p< 0.05.