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. 2020 Nov 16;13(1):910–932. doi: 10.18632/aging.202202

Figure 2.

Figure 2

Tan IIA inhibits LPS- and oxLDL-induced pro-inflammatory cytokine and NLRP3 production in macrophages. (A, B) Effects of Tan IIA on LPS- or oxLDL-induced NF-κB P56 activity in the cytoplasm and nucleus of mouse B6 macrophages. (C) Effects of Tan IIA at the different doses (2.5, 5 and 10 μg/mL) on pro-IL-1β expressions by qRT-PCR detection in LPS- and oxLDL-induced mouse B6 macrophages. (D, E) Effects of Tan IIA on pro-IL-1β expressions detected by Western blot in LPS- and oxLDL-induced B6 macrophages, respectively. (F, G) Effects of Tan IIA on IL-6 expression in the cells by RT-PCR and in the culture medium by ELISA, respectively, in LPS- and oxLDL-induced B6 macrophages. (H, I) Effects of Tan IIA on TNFα expression in the cells by qRT-PCR and in the culture medium by ELISA, respectively, in LPS- and oxLDL-induced B6 macrophages. (J) Effects of Tan IIA on NLRP3 expressions by qRT-PCR detection in LPS- and oxLDL-induced mouse B6 macrophages. (K, L) Effects of Tan IIA on NLRP3 expressions in the culture medium by Western blot in LPS- and oxLDL-induced B6 macrophages, respectively.