Figure 3.
Biochemical characterization of SARS-CoV antibodies for their cross-reactivity with SARS-CoV-2 proteins
Characterization of the S-specific antibodies by western blot and ELISA.
(A) Coomassie stain of in-house-purified His6-tagged RBD protein produced in HEK293-F suspension cells and purified by Ni-NTA chromatography.
(B) Western blot of purified RBD with anti-His6 antibody and SARS-CoV S-specific antibodies.
(C) Ponceau stain of SARS-CoV-2-infected and uninfected Vero E6 cell lysate.
(D) Western blot of SARS-CoV-2-infected lysate probed with SARS-CoV structural protein-specific monoclonal antibodies. Shown are representative images of two to three independent experiments.
(E) Summary table of observed EC50 values from both sets of ELISAs.
(F) ELISA on purified full-length spike coated at 2 μg/mL.
(G) ELISA on purified RBD coated at 2 μg/mL. n = 3 (each done in triplicates). Asterisks indicate an expected alternate band.
See also Figures S3 and S4.