REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibody
Human monoclonal anti-SARS-Cov -S CR3022	BEI Resources	NR-52392
Mouse anti-SARS-CoV S Monoclonal IgM 154C	BEI Resources	NR-620
Mouse anti-SARS-CoV S Monoclonal IgG2a 240C	BEI Resources	NR-616
Mouse anti-SARS-CoV S Monoclonal IgG2a 341C	BEI Resources	NR-617
Mouse anti-SARS-CoV S Monoclonal IgG2a 540C	BEI Resources	NR-618
Mouse anti-SARS-CoV M Monoclonal IgM 19C	BEI Resources	NR-615
Mouse anti-SARS-CoV M Monoclonal IgG1 283C	BEI Resources	NR-621
Mouse anti-SARS-CoV E Monoclonal IgM 472C	BEI Resources	NR-614
Mouse anti-SARS-CoV N Monoclonal IgM 42C	BEI Resources	NR-619
Rabbit anti-SARS-CoV S polyclonal sera-hydrogel	BEI Resources	NRC-773
Rabbit anti-SARS-CoV S polyclonal sera	BEI Resources	NRC-768
Guinea pig anti-SARS-CoV S polyclonal sera	BEI Resources	NR-10316
Mouse anti-2xStrep monoclonal antibody	Sigma Aldrich	SAB2702215
Goat anti-mouse HRP IgG	R & D Systems	HAF007; RRID:AB_357234
Goat anti-mouse HRP IgG	Cell Signaling	7076; RRID:AB_330924
Goat anti-mouse HRP IgM	Invitrogen	62-6820; RRID:AB_138470
Goat anti-rabbit IgG HRP	Cell Signaling	7074; RRID:AB_2099233
Donkey anti-human HRP	Sigma Aldrich	SAB3701359
Goat anti-Llama IgG (H+L) HRP	Novus	NB7242; RRID:AB_10124657
antiHis-HRP	Invitrogen	ma1-80218; RRID:AB_931258
anti-Human-AlexaFluor488	Invitrogen	A11013; RRID:AB_141360
anti-Mouse IgG AlexaFluor555	Invitrogen	A21422; RRID:AB_141822
anti-Mouse IgM AlexaFluor488	Invitrogen	A21042; RRID:AB_2535711
anti-Rabbit FAB2 AlexaFluor555	Invitrogen	4413S; RRID:AB_10694110
Biological samples
Human deidentified Patient Sera 1v6	Messer Lab, Oregon Health & Science University	N/A
Human deidentified Patient Sera 0v1	Messer Lab, Oregon Health & Science University	N/A
SARS-CoV-2 Isolate USA-WA1/2020	BEI Resources	NR-52281
Chemicals and recombinant proteins
RBD recombinant purified Sars-CoV-2 Spike	BEI Resources	NR-52306
Full-length Soluble SARS-CoV-2 Spike	BEI Resources	NR-52308
BSA	Gold-Bio	A-420-10
FBS	VWR	97068-085
PBS	Lonza Biosciences	17-516F
Trypsin	Thermo Fisher Scientific	25200056
TrueBlue	SeraCare	5510-0030
Cell lines
HEK293T	ATCC	CRL-3216
HEK293T human Ace2 expressing stable cell line	BEI Resources	NR-52511
Vero E6	ATCC	VERO C1008
Recombinant DNA
HDM_IDTSpike_fixK, SARS-CoV-2 plasmid	BEI Resources	NR-52514
HDM_Hgpm2	BEI Resources	NR-52517
HDM_tat1b	BEI Resources	NR-52518
pRC_CMV_Rev1b	BEI Resources	NR-52519
pHAGE2_CMV_ZsGreen_W	BEI Resources	NR-52516
pTwist-EF1alpha-nCoV-2019-S-2xStrep	Gordon et al., 2020	N/A
pLVX-EF1alpha-nCoV-2019-E-IRES-Puro	Gordon et al., 2020	N/A
pLVX-EF1alpha-nCoV-2019-M-IRES-Puro	Gordon et al., 2020	N/A
pLVX-EF1alpha-nCoV-2019-N-IRES-Puro	Gordon et al., 2020	N/A
Software
R	Rstudio https://rstudio.com/	https://rstudio.com/
Python	Python Software Foundation	https://www.python.org/
T-Coffee	Notredame et al., 2000	http://tcoffee.crg.cat/
Data Analysis HT(10.0.0.48)	ForteBio	50-5029
Critical commercial assays
PureLink HiPure Plasmid Maxiprep Kit	Invitrogen	K210007
ChromaLINK biotin protein labeling kit	Vector Labs	B-9007-105K
Streptavidin (SA) Biosensors	ForteBio	18-5019
Other
NuncSorp ELISA Plates	Thermo Fisher Scientific	44-2404-21
PVDF membrane	Thermo Fisher Scientific	88518