Fig. 1.

Influence of cold exposure on mechanical and thermal sensitivity in wild type and Scn11a p.R222S mutant mice. a The 50% mechanical withdrawal threshold (mg) in wild type (WT, n = 10) mice and Scn11a p.R222S mutant (R222S, n = 10) mice. b The thermal withdrawal latency (seconds) in WT (n = 7) mice and R222S (n = 7) mice. WT and R222S mutant mice were exposed to cold (4 °C, overnight), and the mechanical/thermal sensitivities within 1.5 h were measured. Naive, mice housed in room temperature (20–23 °C); Cold exposed, mice after cold exposure (4 °C, overnight). Data are presented as the mean ± SEM. Statistical significance was calculated using Welch’s t test. *p < 0.05, ***p < 0.001. NS, not significant