Fig. 3.

Pro-inflammatory mediator levels in plasma and spinal cord samples in wild type and Scn11a p.R222S mice. The levels of pro-inflammatory mediators in plasma (a) and spinal cord (b) samples were determined using a cytokine assay system (Bio-Plex suspension Array System, Bio-Rad Laboratories). Wild type (WT) and Scn11a p.R222S mutant (R222S) mice were divided into four groups (n = 7–10/group): (1) WT naive group, (2) WT cold-exposed group, (3) R222S naive group, and (4) R222S cold-exposed group. Naive, mice housed in room temperature (20–23 °C); Cold exposed, mice after cold exposure (4 °C, overnight). n.d., not detected. Data are presented as the mean ± SEM