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. 2021 Jan 25;11:2158. doi: 10.1038/s41598-020-79607-1

Figure 7.

Figure 7

Both, C5aR1 and C5aR2 knockout PT + HS mice show attenuated BAL IL-6 and IL-17 but do not alter PMN recruitment. BAL samples were tested for (A) number of PMNs, (B) total protein content, (C) IL-6 and (D) IL-17. (E) Plasma CC16 levels. Kruskal–Wallis test was performed with Dunn’s post-hoc analysis, where *, denotes p < 0.05 and *** denotes p < 0.001, to compare the sham group of each genotype with the corresponding PT + HS group and # denotes p < 0.05 when compared with the WT PT + HS mice group. (F–K) Formalin-fixed, paraffin-embedded lung sections of Sham (WT), Sham (C5aR1 KO), Sham (C5aR2 KO), PT + HS (WT), PT + HS (C5aR1 KO) and PT + HS (C5aR2 KO) mice were stained with H&E staining to characterize lung morphology. Intra-alveolar cell infiltration is indicated with a black arrow and RBCs are indicated with a blue arrow. A total of n = 8–12 lungs were stained for each group, and one representative image per group is presented. Images are at 40 × magnification. Bar = 50 µm. (L–N) Formalin-fixed, paraffin-embedded lung sections of PT + HS (WT), PT + HS (C5aR1 KO) and PT + HS (C5aR2 KO) mice stained for MPO to detect neutrophil recruitment and activity within the lungs. Infiltrated granulocytes (marked with black arrow), alveolar epithelial cells and terminal bronchiolar epithelial cells are positively stained for MPO. A total of n = 4 lungs were stained for each group, and one representative image per group is presented. Images are at × 40 magnification. Bar = 100 µm.