Fig. 3. Distinct gene expression profile in post vaccination sorted antigen-specific T_EM and T_CM populations.

a Gene expression heat map identifying uniquely uregulated genes within T_CM and T_EM compartments for all 24 rhesus macaques (red indicates upregulation). b Gene expression profile between 1086.C ENV peptide stimulated and unstimulated cells describing up- and downregulated genes of CD4+ CD154+ populations. c, d The SVM-RFE (Support Vector Machine with Recursive Feature Elimination) transcriptional profiling is shown for T_CM and T_EM compartments based on expression of CD154 in c post prime and d post boost time points. Both T_CM and T_EM cells could be readily discriminated based on CD154 expression.