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. 2021 Jan 12;11:627880. doi: 10.3389/fpls.2020.627880

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Copyright © 2021 Peng, Ma, Lu and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Silencing of N. benthamiana PDS gene by SYNV VIGS vectors. (A) Symptoms and silencing phenotypes of plants infected by SYNV vector expressing a sense (SYNV-sPDS), antisense (SYNV-asPDS), or hairpin RNA (SYNV-hpPDS) of a portion of the PDS gene at 20, 30 and 40 dpsi. (B) qRT-PCR analysis of the relative mRNA levels of PDS in systemically infected leaf tissues at 40 dpi. Data are the means of three independently infected plants (n = 3). Error bars show the standard deviation (± SD). Different letters represent significant differences at p < 0.05 according to one-way ANOVA followed by Tukey’s multiple comparisons test.