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. 2021 Jan 12;11:627880. doi: 10.3389/fpls.2020.627880

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Copyright © 2021 Peng, Ma, Lu and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PDS gene silencing in N. benthamiana by SYNV amiRNA vector. (A) Schematic representation of amiRNA expression and the base pairing between amiRPDS and the target PDS mRNA. m⁷G: cap; A_n: poly(A) tails. miR, microRNA. (B) Phenotypes of plants infected with SYNV and SYNV-amiRPDS at 30 dpsi. (C) End-point stem-loop RT-PCR analysis of mature amiRPDS levels in systemically infected leaf tissue at 30 dpsi. The actin gene was used as an internal control. (D) qRT-PCR analysis of PDS mRNA levels in upper leaf tissues of infected plants at 30 dpsi. Data are the means of three infected plants (n = 3). Error bars show the standard deviation (± SD). Double asterisk (**) indicate significant difference at p < 0.01 according to Student’s t-test.