Table 1.

Misidentification as unidentified ASVs (NA) and ASVs assigned to other genera, the Shannon diversity and richness of bacterial genera as well as the residual sum of squares (RSS) as discrepancy of the sequenced community composition and the structure of the mock community on genus level

Community	Misidentification		Shannon diversity			Richness		RSS to Mock
	NA	Other genera	Mock	Raw	GCN	Mock	Raw	Raw	GCN
Mock-12	0.0003	0.0461	2.0736	0.3926	0.5765	11	13	1.3138	1.3274
Mock-13	0.0005	0.0057	2.8216	2.6968	2.7120	18	32	0.5198	0.5258
Mock-14	0.0003	0.0103	2.8216	2.7039	2.7456	18	35	0.5245	0.4991
Mock-15	0.0001	0.0038	2.8216	2.6950	2.6591	18	30	0.5447	0.5823
Mock-16	0.0853	0.0913	3.7543	3.1574	3.0887	46	54	0.8441	0.9053
Mock-18	0.0019	0.0000	2.7081	2.6027	2.4329	15	15	0.3089	0.5965
Mock-19	0.3074	0.0000	2.3581	2.4581	2.1697	15	15	0.8829	1.1353
Mock-20	0.0000	0.0001	2.7616	2.5335	2.4519	17	17	0.5107	0.5766
Mock-21	0.0000	0.0000	1.6901	1.5246	1.5091	17	14	0.4041	0.3534
Mock-22	0.0004	0.0292	2.7616	2.7212	2.7024	17	20	0.2978	0.4075
Mock-23	0.0008	0.0017	1.6901	1.7205	1.7214	17	20	0.1938	0.1566

The mock community (Mock) was compared with the 16S amplicon sequencing data without (raw) or with gene copy normalization (GCN). Mock-12 was removed from the analysis due to the low Shannon diversity of the sequencing data, accounting for only 20% of the real diversity (shown in red). The best fit of the sequencing data with or without GCN using RSS compared with the mock composition is shown in bold