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. 2021 Jan 25;12(1):121. doi: 10.1038/s41419-021-03416-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A IB analysis of the indicated proteins in control shRNA or YAP1-depleted SW 1353 cells. Cry61 was used as a positive control for the inactivation of YAP1 signaling. B A total of 154 miRNAs were found to be up- or downregulated in YAP1-depleted SW 1353 cells by high-throughput small RNA sequencing. C Eleven p21-regulating miRNAs were identified in YAP1-depleted SW 1353 cells by overlapping the above sequencing data with the predicted p21-targeting miRNAs with the online tools miRDB, miRTarBase, TargetScan, and DIANA-microT. D Heat map of 11 miRNAs regulated by YAP1 in SW 1353 cells. E The consensus sequences targeting p21 in the miR-17 family members were aligned. F qRT-PCR was used to quantify the expression of miR-17, miR-20a, miR-20b, miR-93, miR-106a, and miR-106b in the indicated cells. G–I Control shRNA or YAP1-depleted SW 1353 cells were transfected with either miR-93 Control or miR-93 Mimic, and expression of p21 and p27 was analyzed by IB (G). The percentage of SA-β-gal-positive cells was quantified (H), and representative photos of the senescent cells are shown in (I), scale bars: 20 μm. J Control shRNA or YAP1-depleted SW 1353 cells were transfected with either miR-93 Control or miR-93 Inhibitor, and expression of the indicated protein was detected by IB. Data are presented as the mean ± SD of at least three independent experiments in (F, H). Student’s t-test for (F). Two-way ANOVA followed by Tukey’s test for (H). n.s. nonsignificant, **P < 0.01, ***P < 0.001, ****P < 0.0001.