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. 2021 Jan 12;8:618617. doi: 10.3389/fcell.2020.618617

FIGURE 5.

FIGURE 5

TCONS-00036665 overexpression promotes PSC proliferation but inhibits PSC differentiation. (A) qPCR results showing that TCONS-00036665 expression was significantly increased. (B) Western blotting results showed that Ki67 protein expression was significantly enhanced by TCONS-00036665 overexpression, and relative Ki67 protein expression was quantified by ImageJ software. (C) EdU staining results showing that TCONS-00036665 overexpression remarkably enhanced cell proliferation ability. (D) Western blotting results showing that the protein expression of MyoD, MyoG, and MyHC was significantly inhibited by TCONS-00036665 overexpression, and relative MyoD, MyoG, and MyHC protein expression was quantified by ImageJ software. (E) MyHC immunofluorescence staining results showing that the proportion of MyHC+ was significantly decreased by TCONS-00036665 overexpression. The relative RNA and protein levels were normalized to those of the control β-actin. Error bars represent the mean ± SD of three biological replicates. *p < 0.05, **p < 0.01.