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. 2021 Jan 12;8:618617. doi: 10.3389/fcell.2020.618617

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Copyright © 2021 Wang, Xu, Liu, Jin, Zhu, Bai, Guo, Zhang, Zuo, Xu and Zuo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TCONS-00036665 regulates myogenesis through EZH2. (A) Biotin-labeled full-length TCONS-00036665 was used to pull down EZH2. Western blotting result showed that TCONS-00036665 could interact with EZH2. PCNA is a nucleoprotein used as negative controls. (B,C) Western blotting results showed that p21 protein expression was increased by TCONS-00036665 knockdown (B) but decreased by TCONS-00036665 overexpression (C). Relative p21 protein expression was quantified by ImageJ software. (D,E) ChIP-qPCR results showed that the enrichment of EZH2 (D) and H3K27me3 (E) proteins on the promoters of p21, MyoG, and Myh4 were significantly reduced after TCONS-00036665 knockdown in PSCs. (F,G) ChIP-qPCR results showed that the enrichment of EZH2 (F) and H3K27me3 (G) proteins on the promoters of p21, MyoG, and Myh4 were significantly enhanced after TCONS-00036665 overexpression in PSCs. The relative protein levels were normalized to those of the control β-actin. Error bars represent the mean ± SD of three biological replicates. *p < 0.05, **p < 0.01.