Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 12;8:567358. doi: 10.3389/fcell.2020.567358

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Ashworth, Harrington, Hammond, Bains, Koudouna, Hayes, Ralphs, Regini, Young, Hayashi, Nishida, Hughes and Quantock.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Localization of CS using antibody 7D4 within developing SEAM cultures at weeks 4 and 6 of differentiation. (A) Wide-field view of three SEAM colonies at week 4 of differentiation, two of which are beginning to merge (7D4 is shown in green and nuclear Hoechst-334 staining in blue). (B) Bright-field image of a SEAM at week 4 of differentiation with each zone (Z) indicated. (C) Higher magnification view of CS immunolocalisation in a selected region in (A). (D) High magnification of a SEAM at week 6 of differentiation indicating a widespread deposition of CS. Scale bars = 100 μm. Methods and negative control images with primary antibodies omitted are provided as Supplementary Material.