FIGURE 5.

Observation and examination of the kidney and adrenal glands at 5 dpi. (A–D) The kidney lesions were characterized primarily by coagulation necrosis with occasional detachment of the epithelium from the tubular basement membrane. Some proteinaceous debris was present in the dilated proximal and distal tubules or collecting tubules. (E,F) Low viral RNA expression was also detected by ISH in the renal tubules. (G–I) Scattered inflammatory cells, mainly including neutrophilic cells (black circle in H,I) and lymphocytes (green circle in H), in the adrenal cortex and medulla. (J,K) Robust viral RNA expression was detected by ISH in the adrenal medulla. (L,M) Abundant viral S protein expression was detected by IHC in the cytoplasm. The black frames in the corner of L is the magnification of the framed region. Sequential sections were stained by H&E and subjected to ISH, IHC, and IF. Data are representative of three independent experiments.