FIGURE 1.

C-terminal peptide modifications variably modulate short-range signaling during Drosophila eye development at 18°C. (A) Quantification of ommatidia per eye of female flies shown in (B–L). Statistical significance was determined by one-way ANOVA followed by Sidak’s multiple-comparison test. ***P < 0.001, **P < 0.01, *P < 0.05. ns: not significant. (B) Wild-type fly eyes (w¹¹¹⁸) consist of several hundreds of ommatidia that develop in response to Hh signaling. Scale bar 100 μm. (C) In hh^bar3/hh^AC female flies, eye development was strongly impaired. (D) As a negative control, CD8-GFP expressed in hh^bar3/hh^AC flies under eye disc-specific GMR control did not rescue the hh^bar3/hh^AC loss-of-function phenotype. (E) Expression of untagged Hh and (F–I) C-terminally modified Hh variants rescued eye development in hh^bar3/hh^AC flies to varying degrees. (J,K) Non-cholesteroylated hhN and hhN^GFP partially rescued or fully restored eye development, respectively. (L) Hh^{GFP BAC} expression under endogenous promotor control also fully rescued eye development. All crossings were carried out at 18°C.