Table 2.

LC gradients for RDV, GS-441524 and GS-704277.

RDV			GS-441524			GS-704277			Flow (mL/min)
Time (min)	% Phase A	% Phase B	Time (min)	% Phase A	% Phase B	Time (min)	% Phase A	% Phase B
0	40	60	0	100	0	0	100	0	0.5
0.1	40	60	0.3	100	0	0.2	100	0	0.5
1.6	25	75	2.3	40	60	2	35	65	0.5
1.7	0	100	2.4	0	100	2.1	0	100	0.5
2.6	0	100	3.3	0	100	3	0	100	0.5
2.62	40	60	3.32	100	0	3.02	100	0	0.5
3.4	40	60	4.3	100	0	4	100	0	0.5

Mobile Phase A: 10 mM ammonium formate in 5% methanol, pH 2.5.

Mobile Phase B: 100% methanol.