Table 6.

Stability data established in the method validation.

Analyte Name	GS-5734	GS-441524	GS-704277
Stock Solution Stability in	243 Days at −20 °C	243 Days at −20 °C	70 Days at −20 °C
Acetonitrile:Dimethyl Sulfoxide at 50:50 (v:v)	8 h at RT	8 h at RT	6 h at RT
Processed Sample Stability	145 h at 4 °C	155 h at 4 °C	185 h at 4 °C
Benchtop Stability in Formic Acid-Treated Plasma	8 h in an Ice Bath	8 h in an Ice Bath	8 h in an Ice Bath
Freeze/Thaw Stability in Formic Acid-Treated Plasma	5 Cycles at −20 °C and −70 °C	5 Cycles at −20 °C and −70 °C	5 Cycles at −70 °C
Benchtop Stability in Whole Blood	4 h in an Ice Bath	4 h in an Ice Bath	4 h in an Ice Bath
Long-term Storage Stability in Formic Acid-Treated Plasma	392 Days at −70 °C	392 Days at −70 °C	257 Days at −70 °C
	3 Days at −20 °C	3 Days at −20 °C
Gamma-Ray Irradiation Stability at Required Radiation Dose of 5 Mrad on Dry Ice for Virus load < 1 × 106 TCID50	No impact on assay performance	No impact on assay performance	Data negative bias (impact on assay performance)