FIGURE 9.

BDNF and SIRT-1 in brain homogenates following 14 h of CR. (A) The Western blot was probed first with SIRT1 antibody and showed no difference between CR (n = 3) and control groups (n = 3; p = 0.78) via unpaired t-test. It was then stripped and reprobed with an anti-tubulin antibody to confirm loading equivalence. (B) The Western blot was probed first with BDNF antibody and showed no significant difference between CR (n = 3) and controls (n = 3; p = 0.16) via unpaired t-test. It was then stripped and reprobed with an anti-tubulin antibody to confirm loading equivalence. Lane 1–3 are control samples, Lane 3–6 are CR samples.