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. 2021 Jan 12;11:619404. doi: 10.3389/fpls.2020.619404

FIGURE 1.

FIGURE 1

Anther and meiocyte collection and staging. Anthers were collected according to their size and staged with both acetocarmine (A,C,F,I,K,M) and immuno-cytology (B,D,E,G,H,J,L,N). (A,B) Pre-meiotic stage; (C) leptotene/zygotene stage; (D) leptotene stage; (E) zygotene stage; (F) pachytene stage; (G) pachytene stage; (H) diplotene stage; (I,J) metaphase I; (K,L) anaphase I; (M,N) tetrads; (O,P) isolated fresh meiocyte clusters at leptotene/zygotene and pachytene/diplotene, respectively. Acetocarmine (Gray), DAPI (Blue and White for images O,P), ASY1 (Green), ZYP1 (Magenta). Scale bar 5 μm. Note images (O,P) have associated videos.