4.

各组小鼠S100B表达及脑组织神经炎症水平

Expression of S100B and neurological inflammation levels in different groups of mice. A: Genotyping of gp120 transgenic mice; B, C: Expression of S100B, IL-6 and TNF-α in mice determined by the ELISA; D: Expression of iNOS and RAGE detected by Western blotting; E: Immunohistochemistry for detecting iNOS, RAGE, GFAP and Iba-1 expressions in the cortex and hippocampus (×400). Quantitative analysis of the results of Western blotting and immunohistochemistry staining was performed with Image J grayscale scanning, and the results are presented as Mean±SD (n=3). *P < 0.05, ***P < 0.001 vs gp120 group.