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. 2021 Jan 19;13(1):140. doi: 10.3390/v13010140

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Identification of nucleolar like sequence (NoLS) in NSs proteins. (a) Subcellular localization of 3xFlag-NSs (left panels) and eGFP-NSs (right panels) of PUUV, TULV and PHV was obtained by immunofluorescence performed in VeroE6 cells at day 2 post transfection. 3xFlag-NSs was detected with anti-Flag mAb, then anti-mouse IgG Alexa-488 antibody, while eGFP-NSs was directly fluorescent. (b) shows predicted NoLS of NSs proteins of PUUV, TULV, and PHV determined by NoD software. Scores higher than 0.8 are highlighted in pink. (c) Prediction of naturally disordered NSs regions of the three orthohantaviruses was obtained by in silico analysis with the PONDR^® software and NoLS are highlighted in orange. (d) shows alignment of amino acid sequences of NSs from PUUV, TULV, and PHV, along with conserved amino acid residues (dots and stars below the set of sequences). The predicted NoLS sequence of TULV and the hypothetical one for PHV are highlighted in blue and the conserved polar residues in red.