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Effects of redox reactions on the absorption spectra of 100 μM 1,4-DB (DB) and 100 μM 1,4-BQ (BQ) in room air (21% O2). (A) DB was autoxidized to BQ in 21% O2 as shown by a decrease the absorption peak at 289 nm and an increase in absorption at 249 nm. (B) The BQ absorption peak at 249 nm was unaffected by O2, whereas addition of 100 μM H2S to BQ (C) rapidly decreased the absorbance peak at 249 nm and increased absorbance at 289 nm, indicative of reduction of BQ to DB.
