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. 2021 Jan 19;22(2):979. doi: 10.3390/ijms22020979

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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RNA quality and quantity of equine uterine extracellular vesicles (EVs) after RNase protective assay. EVs samples were treated with RNase (+/−), RNase and Triton X-100 (+/+) or not treated (−/−). (A) RNA was isolated and measured with Nanodrop 3300 fluorometer, Agilent 2100 Bioanalyzer, and Quantus™ fluorometer. Different letters indicate significant differences among RNA treatments (a,b: p < 0.05). Bioanalyzer profiles show RNA quality assessment of not treated EVs samples (B); EVs samples treated with RNase but not Triton X-100 (C); and EVs samples treated with RNase and Triton X-100 before RNA isolation and quantification (D).