Figure 9.
Western blotting analysis of Bax, Bcl-2, Cyt-C, Cas-3 p53 and Ratio (Bax/Bcl-2) in Caco-2 cells. (A) Representative immunoblotting images demonstrate the effect of IC50 dose of ATRA, EC19, and EC23 on the protein expression levels of Bax, Bcl-2, Cyt-C, cleaved Cas-3, and p53 in Caco-2 cells treated for 24 h. (B) Quantification of the tested proteins in Caco-2 cell lysates, with normalization to the β-actin protein (dashed line). The expression of all recorded proteins in the control group is set to (“1”), and all data from three separate experiments are fold change of proteins expression to control (dashed line) and shown as mean ± SEM. * p < 0.05 indicates a statistically significant difference from the matching vehicle-treated control group.