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. 2021 Jan 19;26(2):511. doi: 10.3390/molecules26020511

Figure 2.

Figure 2

Effect of rapamycin and z-VAD-fmk on ADI-PEG 20-induced cell death in MC38 cells. (A) MC38, EMT6, MDA-MB-231, Hep3B, and HT29 cells were examined for ASS1 protein expression by Western blot analysis. Mouse muscle sample was served as ASS1-positive. (B) Caspase 3/7 activity of MC38 cells after 24 h of treatment. (C) MC38 cells were treated with ADI-PEG 20 (0.2 μg/mL) and NAC with or without z-VAD-fmk (50μM) for 48 h. (D) MC38 cells were treated with ADI-PEG 20 (0.025 μg/mL), with or without rapamycin (400 nM) for 48 h, and cell viability was then determined by trypan blue exclusion assay. The data are presented as the mean ± SD from 3 independent experiments. ** p < 0.01 (comparison to the control).