Figure 2.

Characterization of compound 23. (a) Ionic currents recorded in representative oocytes expressing human α7 nAChRs. Currents were evoked by 600 ms applications of ACh 200 μM in the absence (continuous line) and in the presence (dotted line) of 10 μM of 23. All currents were recorded at a holding potential of −80 mV. (b) Concentration–response relationship for the potentiating effect of 23 co-applied with ACh 200 μM. Continuous line represents the fit to the Hill equation. Thus, maximal potentiating effect was estimated as 2.7 ± 0.3-fold, and the EC₅₀ value was 0.44 ± 0.28 μM. Data are taken from 3–7 oocytes from at least 2 donors.