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. 2021 Jan 19;22(2):967. doi: 10.3390/ijms22020967

Figure 2.

Figure 2

NADPH and NADH levels in airway cells. The amounts of NADPH (A-A’) and NADH (B-B’) were assayed as in [24,25]. Cells were incubated for 15 min in the absence or presence of GSNO (1 mmol/L), GLUT (10 mmol/L), ADP (1 mmol/L). Each level value was expressed as % of control, i.e., sample in the absence of compound, to which value 100 was given. Values were subjected to statistical analysis (Wt, n = 5; CF, n = 5): * p < 0.05; ** p < 0.01; *** p < 0.001 when comparing untreated cells with the cells in the presence of compounds. Inset to (A) functionality of mitochondria verified by the ability of (GLUT + ADP) to stimulate oxygen consumption. Inset to (A’) NADPH level measured in Wt cells treated with 6AN (5 mmol/L), GSNO (1 mmol/L), OXA (1 mmol/L), OXM (3 mmol/L).