Figure 4.

Identification of fibroblasts and differentiated keratinocytes within raft structures. Immunofluorescence analysis of tissue sections from all raft setups was performed using antibodies specific to the fibroblast marker α-smooth muscle actin (SMA) (yellow, Column II) and differentiated epithelial marker cytokeratin 10 (K10) (aqua, Column III). Corresponding H&E images are shown in Column I. Note for Setup 6 Panel II, the fluorescent surrounding the MCC lesion is likely background. Each raft setup is shown on the left and cell types are represented using the symbols outlined in Figure 1B. For SMA, 63× images of a positive fibroblast cell (with DAPI counterstain) are included as inset pictures in the top left corner. All scale bars = 100 µM.