Fig. 2.

Decreased tau oligomer levels in synaptosomes from the hippocampus and frontal cortex of NDAN subjects. Western blots of crude synaptosomal fractions from six subjects each of control, AD, and NDAN groups obtained from either (A) the frontal cortex or (C) hippocampus were assessed using Tau 5 antibody. Tau oligomeric species identified in the dotted boxed regions (75 kDa to > 250 kDa) were used to quantify and compare between the three groups. Relative oligomeric tau levels detected by Tau 5 antibody (quantified using β-actin loading controls) show significantly lower levels in control and NDAN (B) frontal and (D) hippocampal synaptosomes compared to AD (^****p < 0.0003, n = 6, Kruskal-Wallis one-way ANOVA, Dunn’s post-hoc).