Fig. 5.

Preserved synaptic potentiation in synaptosomes from hippocampus and frontal cortex of NDAN subjects compared to AD patients. Representative density plots showing GluA1-Nrx1β association and detection in different quadrants in size-gated synaptosomes following basal or cLTP treatment in frontal cortex synaptosomes from Ctrl, AD, or NDAN subjects. Fluorescence thresholds (non-specific) were set by staining with secondary antibodies only (lower left quadrant). Percentage GluA1/Nrx1β events increase in the upper right quadrant was used as a measure of cLTP, relative to basal conditions. Percent basal potentiation for Ctrl (clear bars), AD (black filled bars), and NDAN (grey filled bars) observed in synaptosomes from (A) fontal and (B) hippocampal regions are plotted. Synaptosomes from AD frontal cortex (^*p = 0.0175 compared to Ctrl and ^*p = 0.0283 compared to NDAN, n = 6; one technical repeat, Kruskal-Wallis one-way ANOVA and Dunn’s post-hoc) or hippocampal region (^**p = 0.0043 compared to Ctrl and ^*p = 0.0386 compared to NDAN, n = 6; three technical repeats) did not show potentiation above basal levels and was significantly reduced compared to either Ctrl or NDAN group.