Figure 6.

Effect of pase cyclotides on proliferation of primary human lymphocytes. Human T lymphocytes were left unstimulated and untreated (negative control; NC); all other cells were stimulated with CD3/CD28 mAbs (100 ng/mL) and cultured with medium (positive control; PC), cyclosporin A (CsA; 5 μg/mL), camptothecin (CPT; 300 μM), and different concentrations (1–30 μM) of cyclotides. Cell division analyses were performed by flow cytometry after incubation for 72 h. Data are expressed as the means ± standard deviation (SD) of four independent experiments and were related to untreated stimulated control (PC = 100%). The asterisk (**p < 0.01, ***p < 0.001) represents significant differences from untreated stimulated cells; n.d. means not determined.