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. Author manuscript; available in PMC: 2021 Jan 26.
Published in final edited form as: Methods Mol Biol. 2018;1842:229–239. doi: 10.1007/978-1-4939-8697-2_17

Figure 2. Quantitative and volumetric analyses of GFP and SHG signals in cranial defect healing.

Figure 2.

A bony defect was created in Col2.3GFP transgenic mouse as previously described. The defect healing was imaged over a period of 9 weeks using MPLSM. Propagation of SHG and differentiation of Col2.3GFP (+) osteoblasts were illustrated in panel A (SHG and GFP + cells), B (GFP + cells) and C (SHG, white). Signals of GFP and SHG were isolated within the defect region by proper thresholding across all samples. Volume of GFP (+) cells (D, orange) and SHG (E, cyan) were further calculated based on isolated signals.