Figure 1.

LasB elastolytic activity in culture supernatants from Pseudomonas aeruginosa PAO1 and cystic fibrosis (CF) isolates RP45 and RP73 [wild-type (wt) vs. ∆lasB mutants]. LasB activity in bacterial supernatants (prepared by centrifugation and filtration after 24 h of growth and diluted to 1:10) was evaluated by Elastolytic assay (ECR method, after 20 h of incubation with ECR substrate). Luria-Bertani (LB) growth medium was used as negative control. The data were pooled from two independent experiments in quadruplicate (n = 8 per strain). Statistical significance is indicated as follows: ^*p < 0.05; ^**p < 0.01.