Fig. 3.
Expression of ChIEF-tdTomato by vagal efferent fibers originating from the DVMN, visualized at the cervical level in sheep. (a) Representative cross-section of the whole cervical vagus nerve showing expression of ChIEF-tdTomato (red), Protein Gene Product 9.5 (PGP9.5) immunoreactivity (green) and 4′,6-diamidino-2-phenylindole (DAPI) staining (blue). Scale bar: 500 μm. (b) Photomicrograph of six fascicles taken at a higher magnification showing the expression of ChIEF-tdTomato (red), PGP9.5 immunoreactivity (green) and DAPI staining (blue) merged; bi, bii and biii show individual stains. Scale bars: 100 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)