Figure 5: BRD2 directly regulates transcription of ACE2 and interferon-induced genes.

a, Genes associated with BRD2 CUT&RUN peaks within 10kb of a transcription start site determined in this study in Calu-3 cells overlap significantly with published BRD2 ChIP-seq peaks from the indicated datasets (P < 0.0001, Fisher’s exact test). b, Binding and Expression Target Analysis (BETA) was performed to identify direct BRD2 targets that were differentially expressed upon BRD2 knockdown. ACE2 and several interferon response genes were identified as direct BRD2 targets. c, CUT&RUN recapitulates known BRD2 regulatory sites. Raw signal tracks for IgG, H3K4me3 and BRD2 are shown. Previously reported H3K4me3 CHIP-seq tracks as well as BRD2 CHIP-seq tracks from human lung cells are shown. BRD2 peaks were called over IgG using SEACR at FDR < 0.05. d, A BRD2 CUT&RUN peak was identified upstream of the interferon-induced gene ISG15. Raw signal tracks for IgG, H3K4me3 and BRD2 are shown. BRD2 peaks were called over IgG using SEACR at FDR < 0.05. BRD2 CHIP-seq tracks from human lung cells are also shown. e, A BRD2 CUT&RUN peak was identified within 10 kb upstream of ACE2. Raw signal tracks for IgG, H3K4me3 and BRD2 are shown. BRD2 peaks were called over IgG using SEACR at FDR < 0.05.