(A) CD4+ T cells and from PBMCs were stained for expression of early activation marker CD69 and intracellular cytokine staining (ICS) for IFN γ, IL-4, and IL-2 on day 0 and 7 post challenge. (B) CD8+ T cells from PBMCs were phenotyped for expression of early activation marker CD69 and ICS for IFN γ, IL-4, IL-2, and granzyme B on day 0 and 7 post challenge. (C) NK cell subpopulations were stained for the expression of granzyme B on day 0 and 7 post challenge. Data was measured in duplicate for all animals. Geometric mean and SD are depicted. Statistical significance is indicated.