Table 3:
Inactivation of SARS-CoV-2 in saliva samples diluted with eNAT
| Reagent | Sample matrix | Sample to eNAT ratio | Incubation time (min) | Presence of CPEa (replicates) | Presence of viral RNAb (replicates; Ct±SD) |
|---|---|---|---|---|---|
| eNAT | Saliva with virus | 1:1 | 10 | No (0/3) | Yes (3/3; 41.2±1.1) |
| 15 | No (0/3) | Yes (3/3; 41.1±1.0) | |||
| 30 | No (0/3) | Yes (2/3; 42.3±1.9) | |||
| 1:2 | 5 | No (0/3) | No (0/3) | ||
| 10 | No (0/3) | No (0/3) | |||
| 15 | No (0/3) | No (0/3) | |||
| Tissue culture media without the virus | 1:1 | None | No (0/2) | No (0/2) | |
| 1:2 | None | No (0/2) | No (0/2) | ||
| AVL buffer + heating at 92°C (Negative control) | Virus in tissue culture media | None | 15 | No (0/2) | No (0/2) |
| None (Positive control) | Virus in Tissue culture media | None | None | Yes | Yes (13.8) |
a
Tested by both culture and TCID50;
b
Tested by Xpert Xpress SARS-CoV-2 test