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. 2021 Jan 25;20:24. doi: 10.1186/s12934-021-01514-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Expression and characterization of the PPV VP2 protein in K. marxianus. a Illustration of the recombinant expression plasmid pUKDN115-PPV VP2. The inulinase promoter, inulinase terminator, and URA3 cassette in pUKDN115 are all derived from K. marxianus, and autonomously replicating sequence is from pKD1. b TEM scan of the assembled PPV VLPs in K. marxianus intracellularly. SDS-PAGE (c) and Western Blot (b) assays of the lysates of recombinant strain after shaked for 66 h. Lane M: PageRuler Prestained Protein Ladder;Lane 1: the lysate of Fim-1 ∆ura3 transformed with the empty plasmid pUKDN115; Lane 2: the lysate of KM-PPV-VP2; Lane 3: the supernatant of KM-PPV-VP2 cell lysate; Lane 4: the precipitate of KM-PPV-VP2 cell lysate