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High cell-density fermentation of the KM-PPV-VP2 strain. a The growth curve of KM-PPV-VP2 in a 5L fermentor. b SDS-PAGE of the VP2 protein expression in KM-PPV-VP2 cell lysates at the indicated time. Yeast cells were collected at an interval of 6 h, and cells cultures were then diluted with PBS at a rate of 1:10. After disruption by high-pressure homogenization, each of 15 µl cell lysates was loaded to analyze by SDS-PAGE analysis
