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. 2021 Jan 25;20:24. doi: 10.1186/s12934-021-01514-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Scale-up purification of PPV VLPs. a SDS-PAGE analysis of the purified PPV VLPs samples from IEX and Gel filtration. Lane M, PageRuler Prestained Protein Ladder; Lane 1, cell lysates prepared by PBS pH 7.4; lane 2, the supernatant of cell lysates adjusted to pH 4.0; Lane 3, elution fraction of Capto S ImpAct exchange chromatography; Lane 4, elution of Sephacryl® S-500 Gel filtration. b TEM scan of the Gel filtration purified PPV VLPs. Bar, 100 nm. c HPLC analysises of the supernatant pH adjusted cell lysate (c), the IEX purified PPV VLPs (d), and the gel filtration purified PPV VLPs (e)