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. 2021 Jan 25;20:24. doi: 10.1186/s12934-021-01514-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — SDS-PAGE (a) and HPLC (b) assays of the purified PPV VLPs remained in the pH adjusted precipitates. The precipitates were dissolved using 20 mM Tris-HCl buffer pH 8.0. After clarification by centrifugation, supernatants were loaded onto a Capto Q XP column and PPV VLPs were eluted with 20 mM Tris-HCl pH 7.4 containing 0.5 M NaCl. Elution fractions were ultrafiltrated with a 750 kDa column in a tangential flow filtration. Lane RS: the redissolved supernatant; Lane F: flowthrough fractions; Lane E: elution fractions; Lane U; the ultrafiltrated VLPs