Fig. 3.

LINC01116 accelerates CRC cell proliferation and angiogenesis by mediating TPM1 Note: The expression of TPM1 in CRC tissues of TCGA dataset was searched through GEPIA database (a). The levels of TPM1 in CRC cells were tested by qRT-PCR (b) and Western blot (c). After transfection of pcDNA3.1-LINC01116 or sh-LINC01116 into SW480 and HCT116 cells, qRT-PCR (d) and Western blot (e) were applied to test the levels of TPM1. After SW480 and HCT116 cells were transfected with sh-TPM1, the levels of TPM1 were tested by qRT-PCR (f) and Western blot (g). Cell viability and proliferation were assessed by CCK-8 (h) and colony formation assays (i). Cell scratch (j) and tube formation assays (k) were used to measure migration and angiogenesis. *P < 0.05, **P < 0.01; CRC colorectal cancer, TPM1 tropomyosin 1