Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 26;19:45. doi: 10.1186/s12967-021-02707-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 4 — LINC01116 recruits EZH2 to suppress TPM1 gene transcription. The binding of LINC01116 and EZH2 were predicted by RNAInter online tools (a). The potential binding of EZH2 and TPM1 was predicted by UCSC (b). TCGA database was used to assess the expression of EZH2 in CRC tissues and the association between EZH2 and TPM1 (c). qRT-PCR (d) and Western blot (e) were applied to measure the levels of EZH2 in CRC cell lines. RIP assay confirmed the interaction between LINC01116 and EZH2 (f). Binding of EZH2 to TPM1 promoter was verified by ChIP (g). The augment of TPM1 promoter enriched in EZH2 antibody following overexpression of LINC01116 was detected by ChIP (h). RNA pull-down assay was performed to measure H3K27me3 in LINC01116-complex following overexpression of LINC01116 (i). *P < 0.05, **P < 0.01, ***P < 0.001; TPM1 tropomyosin 1, CRC colorectal cancer