Fig. 5.

MiR-568 targets to AKT3 in HCC to regulate stemness and chemosensitivity. a. Heatmap summarizing the differentially expressed genes in miR-568 overexpressed SNU-449 cells. b. KEGG analysis for enriched pathways in miR-568 overexpressed SNU-449 cells. c. The potential binding between miR-568 and ATK3 sequences predicted by TargetScan. d. The relative luciferase activities in 293 T cells co-transfected with miR-568 mimics or miR-NC and luciferase reporter vectors AKT3-WT or AKT3-Mut. e. Regression curve with respect to expression of miR-568 and AKT3. f. Expression of AKT3 mRNA after knockdown or overexpression of miR-568. g. Expression of AKT3 protein after knockdown or overexpression of miR-568. h. Expression of stemness biomarkers in SNU-449 cells co-transfected with miR-568 mimics or miR-568 mimics NC and AKT3 or AKT3-vec. i. Sphere formation capacities of SNU-449 cells after co-transfection with miR-568 mimics or miR-568 mimics NC and AKT3 or AKT3-vec. j. Analysis of SNU-449 cell viability after co-transfection with miR-568 mimics or miR-568 mimics NC and AKT3 or AKT3-vec under different concentrations of 5-Fu. ^**P < 0.01