Fig. 2.

(a) Chemical structure PLL-g-Dex. (b) Effect of copolymer and LNA modification on substrate cleavage activity. Black and blue lines represent the activities of Mz(7,8) and Lz4(7,8), respectively. Representative cleavage kinetics of each condition were shown. (c, d) Temperature dependence of MNAzymes and ^LMNAzymes with (c) long and (d) short S-arms. Black lines (circle), red line (triangle), and blue line (square) are data for Mz(X,Y), Lz3(X,Y), and Lz4(X,Y), respectively. Dashed lines (open symbols) and solid lines (filled symbols) indicate reactions without and with copolymer, respectively. Reactions contained 100 nM substrate, 20 nM partzyme, and 20 nM miR-21. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)