Fig. 4.

Effect of total RNA from HeLa cells on the activity of MNAzymes with (a) long S-arms and (b) short S-arms. Reaction solutions contained 100 nM substrate, 20 nM partzymes, 1 nM miR-21, without or with HeLa cell total RNA at concentration 5 μg/mL in total reaction volume of 2 mL. MNAzyme assay were conducted in the presence of copolymer at optimal temperature. Experiments were repeated three times.