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. 2021 Jan 25;220(3):e202005153. doi: 10.1083/jcb.202005153

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© 2021 Ito et al.

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Figure 3. — Precociously disengaged centrioles convert into centrosomes during the G₂ phase and mitosis. (A) A portion of disengaged daughter centrioles lack POC5. HeLa cells were treated with siControl or siCep57/Cep57L1 and immunostained with antibodies against centrin (green) and POC5 (red). Values are mean percentages ± SD from three independent experiments (n = 30 for each experiment). (B) Histograms represent the frequency of cells with the indicated number of POC5-positive centrioles among cells with two pairs of centrioles (siControl) or disengaged four centrioles (siCep57/Cep57L1) observed in A. Values are mean percentages ± SD from three independent experiments (n = 30 for each experiment). (C) PCNT was recruited to disengaged daughter centrioles mainly in the G₂ phase and mitosis. HeLa-GFP-centrin1 cells were treated with siCep57/Cep57L1 and immunostained with antibodies against GFP (green), PCNT (red), and CENP-F (cyan). White and black arrowheads indicate PCNT-positive and PCNT-negative centrioles, respectively. (D) Histograms represent the frequency of cells with more than two PCNT-positive centrioles among cells with disengaged four centrioles before, during, and after the G₂ phase observed in C. Values are mean percentages ± SD from three independent experiments (n = 30 for each experiment). (E) Schematic illustration of the results in C and D. PCNT was recruited into disengaged daughter centrioles mainly in the G₂ phase and mitosis. All scale bars, 5 µm in the low-magnification view, 1 µm in the inset.